AN ULTRASTRUCTURAL STUDY OF GLOMERULAR PERMEABILITY USING CATALASE AND PEROXIDASE AS TRACER PROTEINS
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چکیده
منابع مشابه
An Ultrastructural Study of Glomerular Permeability Using Catalase and Peroxidase as Tracer Proteins
Mice were injected intravenously with beef liver catalase (mol wt 240,000) and very small doses of horseradish peroxidase (mol wt 40,000) and the site of localization of these enzymes in the kidney was studied by ultrastructural cytochemistry. 1 min after injection, catalase was present in glomerular capillary lumina and there was minimal permeation of the basement membrane. After 5-180 min, st...
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Beef liver catalase (mol wt 240,000) was injected intravenously into normal rats and rats made nephrotic with aminonucleoside of puromycin. The localization of the tracer in the kidneys was then studied by ultrastructural cytochemistry, 3 min-12 hr after injection. Passage of catalase into the urinary space in normal rats was restricted by the basement membrane and by the epithelial slit pore. ...
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Physiological studies (1-4) have indicated that increased glomerular permeability is the major cause of proteinuria in human and experimental nephrosis, but the ultrastructural basis for such protein leakage is poorly understood. Previous morphological studies using ferritin (tool wt 500,000) (5, 6) have shown that the glomerular basement membrane becomes more permeable to this particle in expe...
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The permeability of the alveolar-capillary membrane to a small molecular weight protein, horseradish peroxidase (HRP), was investigated by means of ultrastructural cytochemistry. Mice were injected intravenously with HRP and sacrificed at varying intervals. Experiments with intranasally instilled HRP were also carried out. The tissue was fixed in formaldehyde-glutaraldehyde fixative. Frozen sec...
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The transendothelial passage of horseradish peroxidase, injected intravenously into mice, was studied at the ultrastructural level in capillaries of cardiac and skeletal muscle. Peroxidase appeared to permeate endothelial intercellular clefts and cell junctions. Abnormal peroxidase-induced vascular leakage was excluded. Neutral lanthanum tracer gave similar results. The endothelial cell junctio...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1970
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.132.6.1153